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Methylene Blue vial, Diluent
Cellular & Longevity

Buy Methylene Blue UK

Methylthioninium chloride, a phenothiazine dye, as a 10mg vial. Research use only.

from £44.991 option

  • In stock Ships from the UK
  • Same-day dispatch Order before 2pm
  • Tracked delivery Free over £75
  • Certificate of analysis On request

Methylene Blue is on the shelf at diluent.co.uk, held in British stock rather than ordered in, priced from £44.99 for the 10mg vial. It is supplied as vial. A weekday order placed before 2pm goes out the same day, tracked, boxed with nothing on the outside about the contents.

Shipping from inside the country is what removes the customs step: no clearance, no import charge, nothing sitting at a border. Purity is ≥99% by HPLC on every batch, and the certificate for the lot you are sent is yours on request against the number on the label.

Delivery runs £4.95, free above £75, tracked start to finish. See how ordering works.

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What is Methylene Blue?

Properly methylthioninium chloride, a phenothiazine dye that cycles between redox states. No peptide bond anywhere in it. Laboratories have used it as a stain for well over a century.

Specification

ClassPhenothiazine dye
Also listed asMethylthioninium chloride, Basic blue 9
Molecular formulaC16H18ClN3S
Molecular weight319.9
CAS61-73-4
Purity≥99% by HPLC
Vial strength10mg
LightStrongly photosensitive, store out of direct light
CertificateOn request, against the batch number

What has Methylene Blue been studied for?

The record behind Methylene Blue is pre-clinical throughout. Not one of the papers listed below is a human trial. Below is what each paper set out to examine, sorted by the model it ran in:

Reviews of the literature

Other published work

  • How worthwhile is methylene blue as a treatment of malaria?, Not stated in the record (PMID 31237457)

Each line describes what a paper looked at. None of it reports an effect in a person, and no part of this site tells anyone how to use anything.

Where and how to buy Methylene Blue in the UK

How do you order?

Pick a strength from the table, put it in the basket and check out. There is no account to set up. The confirmation reaches you straight away and the payment details follow by email, because no card is taken on the site.

Why does UK stock matter?

The Methylene Blue you order leaves a warehouse in Britain. That means no customs step, no import charge and no days lost in transit from abroad. Before 2pm on a working day and it is collected that afternoon, tracked, in an outer box that gives nothing away.

What arrives in the box?

Purity≥99%, by HPLC
LabellingCompound, strength, format, purity, research-use statement and a batch number
Certificate of analysisOn request, matched to your batch number
DeliveryTracked, UK only. £4.95, free over £75
Returns14 days on unopened, sealed items, terms

Reconstituting Methylene Blue

The vial arrives as a freeze-dried cake. It becomes a solution once bacteriostatic water goes in, and how much you add is what decides the strength of that solution. The quantity in the vial never changes, only the volume it is spread through.

5.00 mg/mlConcentration
0.100 mlHolds 0.5mg
10.0 unitsOn a U-100 syringe
50 mcgPer unit of that syringe

What you see here are concentrations and volumes. Nothing on this site is dosage, protocol or administration guidance, and no number above is one either.

Loaded with 10mg and 2ml to start. Move either control and the numbers move with it. The full reconstitution calculator goes further, into syringe units, which water to pick and how long a mixed vial lasts.

Concentration by vial size and water added
Vial+ 1ml+ 2ml+ 3ml+ 5ml
10mg 10 mg/ml5 mg/ml3.33 mg/ml2 mg/ml

Take the 10mg vial: 2ml of bacteriostatic water puts it at 5 mg/ml. The sum is the milligrams on the label over the millilitres you put in. Every figure here is a concentration. None of them is a dose, and this site carries no dosage, protocol or administration guidance at all.

How should it be handled?

  • Let a refrigerated vial reach room temperature first.
  • Wipe the stopper with an alcohol swab every time you go in.
  • Aim the water at the glass wall so it runs down, not straight onto the cake.
  • Roll it gently between your fingers. Shaking makes foam, and foam is damaged protein.
  • Give it a few minutes to go into solution. Done properly it finishes clear.

Storing Methylene Blue

Sealed vialSomewhere cool, dry and dark. For anything long term, the freezer.
Once reconstitutedFridge, standing upright, away from light. Never freeze it once mixed.
LightVery sensitive to light. Keep it dark.

How long does it keep?

General figures for a lyophilised peptide
Stored asKeeps for
Sealed, frozen at −20 °CYears. Nothing holds a lyophilised peptide better than this
Sealed, refrigerated at 2–8 °CMonths
Sealed, at room temperatureWeeks. Fine while it is in the post, not a place to keep it
Reconstituted, refrigeratedThink weeks, not months. Four weeks is the number most people work to once bacteriostatic water is in, which is exactly what its preservative is there for
Reconstituted, frozenBest avoided. Ice crystals tear at peptides, and every freeze–thaw round makes it worse

Treat these as the usual numbers for lyophilised peptides, not a stability result measured on one batch. Cold and dry buys time; moisture, heat, light and being shaken about take it away. Ask and we will send the batch certificate of analysis.

We sell Methylene Blue as an unlicensed research compound, for laboratory research use only. There is no UK marketing authorisation behind it, it is not a licensed medicine, and it is not for human use, veterinary use or food.

Nothing of this kind is a controlled drug, and selling it as laboratory material is lawful. Selling or marketing it for people to take is not. That line is the reason you will not find a dose, a protocol, a route, a frequency or a medical claim anywhere on this site, and the reason asking for one by email gets the same answer.

A few molecules in this range also appear inside a licensed medicine sold under a brand name. That authorisation belongs to the finished product it was granted for; it does not follow the molecule anywhere else. Full terms.

Research use only. Diluent supplies compounds for laboratory research. Nothing we sell is a licensed medicine, we are not a pharmacy, and we publish no dosage, protocol or administration guidance. Not for human or veterinary use, and not for use in food.

Methylene Blue questions

Is methylene blue a peptide?

It is not. This is a dye, from the phenothiazine family.

How should it be stored?

Somewhere dark. Light affects it strongly.

Where can I buy Methylene Blue in the UK?

Methylene Blue is available in the UK from diluent.co.uk, from £44.99, a single 10mg size, as vials. British stock on a British courier. Nothing to pay on import, nothing to handle, nothing for an officer to open. That lands at £4.50 the milligram. It turns up as freeze-dried powder, so the concentration is yours to set when you mix it. Get the order in before 2pm on a weekday and it leaves that day, tracked, plainly packaged. Batches go to a third-party lab and come back at ≥99% by HPLC, and the certificate for yours is there if you ask, see how to order.

How much does Methylene Blue cost?

It starts at £44.99 for the 10mg, £4.50 per mg. Postage runs £4.95, and nothing at all above £75.

How much bacteriostatic water do I add to Methylene Blue?

There is no single right answer, because the volume you choose is what fixes the strength. Take the 10mg vial: 1ml puts it at 10 mg/ml, 2ml at 5 mg/ml, 3ml at 3.33 mg/ml. The 10mg in the vial stays 10mg regardless. These are concentrations. They are not doses.

How should Methylene Blue be stored?

Still sealed and dry it will sit for years at −20 °C, months in a fridge at 2–8 °C, weeks at room temperature. Cold and dry is what buys the time. After mixing it goes in the fridge standing up, and four weeks is the figure most work to with bacteriostatic water. Never freeze it once mixed: ice crystals tear peptides apart and each freeze–thaw round does it again.

What purity is your Methylene Blue, and can I see a certificate?

≥99%, measured by HPLC. Quote the batch number on your label and we will send the certificate of analysis for that lot. If you would rather see where current stock sits before you commit, ask and we will tell you.

References

  1. Methylene blue. American journal of therapeutics, 2003.. PMID 12845393
  2. [Methylene blue]. PMID 19069129
  3. How worthwhile is methylene blue as a treatment of malaria?. Expert review of anti-infective therapy, 2019.. PMID 31237457

Each one links out so you can read the paper itself.